Dr. Coba uses mass spectrometry to find the complete set of proteins that interact directly with another protein. For Syngap1, he has made beautiful and detailed networking diagrams illustrating which proteins are found directly with Syngap1. The list is long, and in fact Syngap1 is a hub of sorts. By studying these interactions over different times in development, and in different cell types, he will draw a more complete picture of all the potential direct roles of Syngap1 within a developing organism.
If that isn’t enough complexity, Dr. Coba studies the exact mutations of patients by collecting blood samples, makes neurons in tissue culture (from iPSCs aka induced pluripotent stem cells), and looks at the effects of genetic background and pathogenic variants on the phenotype. In addition, Dr. Coba currently uses an advanced machine in his lab purchased with a grant from SRF, and will be getting SRF funding for a post-doctoral fellow in 2021. #SRFCoba